Genetic mapping reveals a candidate gene CmoFL1 controlling fruit length in pumpkin

Genetic mapping reveals a candidate gene CmoFL1 controlling fruit length in pumpkin

Blog Article

Fruit length (FL) is an important economical trait that affects fruit yield and appearance.Pumpkin (Cucurbita moschata Duch) contains a wealth genetic variation Organizers in fruit length.However, the natural variation underlying differences in pumpkin fruit length remains unclear.

In this study, we constructed a F2 segregate population using KG1 producing long fruit and MBF producing short fruit as parents to identify the candidate gene for fruit length.By bulked segregant analysis (BSA-seq) and Kompetitive Allele-Specific PCR (KASP) approach of fine mapping, we obtained a 50.77 kb candidate region on chromosome 14 associated with the fruit length.

Then, based on sequence variation, gene expression and promoter activity analyses, we identified a candidate gene (CmoFL1) encoding E3 ubiquitin ligase in this region may account for the variation of fruit length.One SNP variation in promoter of CmoFL1 changed the GT1CONSENSUS, and DUAL-LUC assay revealed that this variation significantly affected the promoter activity of CmoFL1.RNA-seq D analysis indicated that CmoFL1 might associated with the cell division process and negatively regulate fruit length.

Collectively, our work identifies an important allelic affecting fruit length, and provides a target gene manipulating fruit length in future pumpkin breeding.